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plasmid pmal c2  (New England Biolabs)


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    New England Biolabs plasmid pmal c2
    Plasmid Pmal C2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 3339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pmal+c2/pMAL/pmc12796736-249-10-12
    Average 96 stars, based on 3339 article reviews
    plasmid pmal c2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Engineered Escherichia coli Silver-Binding Periplasmic Protein That Promotes Silver Tolerance
    Article Snippet: .. Plasmid pMal-c2 (New England BioLabs), which encodes a signal sequence-less version of E. coli maltose-binding protein (MBP) followed by a multiple cloning site, a polypeptide linker, and the LacZα peptide, was manipulated as described previously ( 27 ) with the following modification. .. Two phosphorylated primers (Invitrogen) encoding an SSSGGG linker peptide followed by the AgBP2 peptide, together with extensions compatible with the overhangs generated upon SacI and XbaI digestion (5′-CG GGC GGT GGC GAA CAA CTG GGC GTG CGC AAA GAA CTG CGC GGC GTG TGA TGA T-3′ and 5′-CT AGA TCA TCA CAC GCC GCG CAG TTC TTT GCG CAC GCC CAG TTG TTC GCC ACC GCC CGA GCT-3′), were annealed and ligated to SacI- and XbaI-digested plasmid.

    Article Title: The Intimin periplasmic domain mediates dimerisation and binding to peptidoglycan.
    Article Snippet: .. The MBP coding sequence was amplified from the plasmid pMal-c2 (New England Biolabs) with primers that © 2014 Max Planck Society. ..

    Article Title: Binding of the brain G protein G⍺ o to its potential effector RASA3 is promoted by Ca 2 .
    Article Snippet: .. A human RASA3 cDNA was cloned into a derivative of plasmid pMAL-c2 (New England Biolabs, NEB) to generate a plasmid (pHSB3) that expresses a fusion protein consisting of N-terminal maltose binding protein (MBP) followed by a TEV cleavage site, full-length RASA3, and a C-terminal His6 tag (pHSB4). ..

    Article Title: Site-specific and effective immobilization of proteins by Npu DnaE split-intein mediated protein trans-splicing reaction
    Article Snippet: Immobilized proteins on solid supports provide a useful platform for various biological assays including proteomics research, protein-protein interaction studies, and diagnosis.. In fabricating protein chips, proteins should be immobilized to maintain activity and specificity towards their targets.. Here, we describe an approach to attach a protein of interest onto a solid support through a covalent bond as well as in situ monitoring of protein immobilization and subsequent binding assays.

    Article Title: Functional interconnections of Arabidopsis exon junction complex proteins and genes at multiple steps of gene expression.
    Article Snippet: .. The AtPYM coding sequence was cloned in-frame with the maltose-binding protein (MBP) in the EcoRI and PstI sites of plasmid pMAL-c2 (New England Biolabs), using oligonucleotides Pym-pMALF and Pym-pMALR. ..

    Article Title: Cancer-testis Antigen OY-TES-1 Expression and Immunogenicity in Hepatocellular Carcinoma.
    Article Snippet: Bin LUO, E-mail: glbinbin2002@yahoo.com; Xiang YUN, E-mail:bnfeiyun@aliyun.com; Jing LI, E-mail: lijing046352 @163.com †The Authors contributed equally to this work.. #Corresponding authors, Wei-xia NONG, E-mail: weixianong @hotmail.com; Qing-mei ZHANG, E-mail: zhangqingmei2017 @outlook.com; Xiao-xun XIE, E-mail: xiaoxunxie@hotmail. com *This work was supported by grants from the National Natural Science Foundation of China (No. 81860445, No. 81960453, No. 81560408, and No. 81660429), Natural Science Foundation of Guangxi Province (No. 2016GXNSFBA380159, No. 2018GXNSFAA281251, No. 2018GXNSFAA050151, No. 2017GXNSFAA198001, No. 2018GXNSFAA281050, and No. 2018GXNSFBA281187), Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University) and Ministry of Education (No. GK201809, No. GKE 2019-08, and No. K2015-TKF03), and Basic Ability Improvement Project for Young and Middle-aged Teachers in Colleges and Universities of Guangxi Province (No. 2018KY0109).. Cancer-testis Antigen OY-TES-1 Expression and Immunogenicity in Hepatocellular Carcinoma*

    Article Title: Binding of the brain G protein G⍺ o to its potential effector RASA3 is promoted by Ca 2+
    Article Snippet: .. A human RASA3 cDNA was cloned into a derivative of plasmid pMAL-c2 (New England Biolabs, NEB) to generate a plasmid (pHSB3) that expresses a fusion protein consisting of N-terminal MBP followed by a TEV cleavage site, full-length RASA3, and a C-terminal His 6 tag (pHSB4). ..

    Sequencing:

    Article Title: Engineered Escherichia coli Silver-Binding Periplasmic Protein That Promotes Silver Tolerance
    Article Snippet: .. Plasmid pMal-c2 (New England BioLabs), which encodes a signal sequence-less version of E. coli maltose-binding protein (MBP) followed by a multiple cloning site, a polypeptide linker, and the LacZα peptide, was manipulated as described previously ( 27 ) with the following modification. .. Two phosphorylated primers (Invitrogen) encoding an SSSGGG linker peptide followed by the AgBP2 peptide, together with extensions compatible with the overhangs generated upon SacI and XbaI digestion (5′-CG GGC GGT GGC GAA CAA CTG GGC GTG CGC AAA GAA CTG CGC GGC GTG TGA TGA T-3′ and 5′-CT AGA TCA TCA CAC GCC GCG CAG TTC TTT GCG CAC GCC CAG TTG TTC GCC ACC GCC CGA GCT-3′), were annealed and ligated to SacI- and XbaI-digested plasmid.

    Article Title: The Intimin periplasmic domain mediates dimerisation and binding to peptidoglycan.
    Article Snippet: .. The MBP coding sequence was amplified from the plasmid pMal-c2 (New England Biolabs) with primers that © 2014 Max Planck Society. ..

    Article Title: Functional interconnections of Arabidopsis exon junction complex proteins and genes at multiple steps of gene expression.
    Article Snippet: .. The AtPYM coding sequence was cloned in-frame with the maltose-binding protein (MBP) in the EcoRI and PstI sites of plasmid pMAL-c2 (New England Biolabs), using oligonucleotides Pym-pMALF and Pym-pMALR. ..

    Cloning:

    Article Title: Engineered Escherichia coli Silver-Binding Periplasmic Protein That Promotes Silver Tolerance
    Article Snippet: .. Plasmid pMal-c2 (New England BioLabs), which encodes a signal sequence-less version of E. coli maltose-binding protein (MBP) followed by a multiple cloning site, a polypeptide linker, and the LacZα peptide, was manipulated as described previously ( 27 ) with the following modification. .. Two phosphorylated primers (Invitrogen) encoding an SSSGGG linker peptide followed by the AgBP2 peptide, together with extensions compatible with the overhangs generated upon SacI and XbaI digestion (5′-CG GGC GGT GGC GAA CAA CTG GGC GTG CGC AAA GAA CTG CGC GGC GTG TGA TGA T-3′ and 5′-CT AGA TCA TCA CAC GCC GCG CAG TTC TTT GCG CAC GCC CAG TTG TTC GCC ACC GCC CGA GCT-3′), were annealed and ligated to SacI- and XbaI-digested plasmid.

    Modification:

    Article Title: Engineered Escherichia coli Silver-Binding Periplasmic Protein That Promotes Silver Tolerance
    Article Snippet: .. Plasmid pMal-c2 (New England BioLabs), which encodes a signal sequence-less version of E. coli maltose-binding protein (MBP) followed by a multiple cloning site, a polypeptide linker, and the LacZα peptide, was manipulated as described previously ( 27 ) with the following modification. .. Two phosphorylated primers (Invitrogen) encoding an SSSGGG linker peptide followed by the AgBP2 peptide, together with extensions compatible with the overhangs generated upon SacI and XbaI digestion (5′-CG GGC GGT GGC GAA CAA CTG GGC GTG CGC AAA GAA CTG CGC GGC GTG TGA TGA T-3′ and 5′-CT AGA TCA TCA CAC GCC GCG CAG TTC TTT GCG CAC GCC CAG TTG TTC GCC ACC GCC CGA GCT-3′), were annealed and ligated to SacI- and XbaI-digested plasmid.

    Amplification:

    Article Title: The Intimin periplasmic domain mediates dimerisation and binding to peptidoglycan.
    Article Snippet: .. The MBP coding sequence was amplified from the plasmid pMal-c2 (New England Biolabs) with primers that © 2014 Max Planck Society. ..

    Clone Assay:

    Article Title: Binding of the brain G protein G⍺ o to its potential effector RASA3 is promoted by Ca 2 .
    Article Snippet: .. A human RASA3 cDNA was cloned into a derivative of plasmid pMAL-c2 (New England Biolabs, NEB) to generate a plasmid (pHSB3) that expresses a fusion protein consisting of N-terminal maltose binding protein (MBP) followed by a TEV cleavage site, full-length RASA3, and a C-terminal His6 tag (pHSB4). ..

    Article Title: Functional interconnections of Arabidopsis exon junction complex proteins and genes at multiple steps of gene expression.
    Article Snippet: .. The AtPYM coding sequence was cloned in-frame with the maltose-binding protein (MBP) in the EcoRI and PstI sites of plasmid pMAL-c2 (New England Biolabs), using oligonucleotides Pym-pMALF and Pym-pMALR. ..

    Article Title: Binding of the brain G protein G⍺ o to its potential effector RASA3 is promoted by Ca 2+
    Article Snippet: .. A human RASA3 cDNA was cloned into a derivative of plasmid pMAL-c2 (New England Biolabs, NEB) to generate a plasmid (pHSB3) that expresses a fusion protein consisting of N-terminal MBP followed by a TEV cleavage site, full-length RASA3, and a C-terminal His 6 tag (pHSB4). ..

    Binding Assay:

    Article Title: Binding of the brain G protein G⍺ o to its potential effector RASA3 is promoted by Ca 2 .
    Article Snippet: .. A human RASA3 cDNA was cloned into a derivative of plasmid pMAL-c2 (New England Biolabs, NEB) to generate a plasmid (pHSB3) that expresses a fusion protein consisting of N-terminal maltose binding protein (MBP) followed by a TEV cleavage site, full-length RASA3, and a C-terminal His6 tag (pHSB4). ..

    Polymerase Chain Reaction:

    Article Title: Cancer-testis Antigen OY-TES-1 Expression and Immunogenicity in Hepatocellular Carcinoma.
    Article Snippet: Bin LUO, E-mail: glbinbin2002@yahoo.com; Xiang YUN, E-mail:bnfeiyun@aliyun.com; Jing LI, E-mail: lijing046352 @163.com †The Authors contributed equally to this work.. #Corresponding authors, Wei-xia NONG, E-mail: weixianong @hotmail.com; Qing-mei ZHANG, E-mail: zhangqingmei2017 @outlook.com; Xiao-xun XIE, E-mail: xiaoxunxie@hotmail. com *This work was supported by grants from the National Natural Science Foundation of China (No. 81860445, No. 81960453, No. 81560408, and No. 81660429), Natural Science Foundation of Guangxi Province (No. 2016GXNSFBA380159, No. 2018GXNSFAA281251, No. 2018GXNSFAA050151, No. 2017GXNSFAA198001, No. 2018GXNSFAA281050, and No. 2018GXNSFBA281187), Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University) and Ministry of Education (No. GK201809, No. GKE 2019-08, and No. K2015-TKF03), and Basic Ability Improvement Project for Young and Middle-aged Teachers in Colleges and Universities of Guangxi Province (No. 2018KY0109).. Cancer-testis Antigen OY-TES-1 Expression and Immunogenicity in Hepatocellular Carcinoma*



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